Role of interactions between SpoIIAA and SpoIIAB in regulating cell-specific transcription factor σF of Bacillus subtilis

Abstract

Genetic experiments have suggested that σF, the first compartment-specific transcription factor in sporulating B. subtilis, is regulated by an anti-σ factor SpoIIAB and an anti-anti-σ factor SpoIIAA. Previously, we reported biochemical results demonstrating that SpoIIAB is both a phosphokinase whose substrate is SpoIIAA and an inhibitor of σF-directed transcription. We now show that in the presence of SpoIIAB and ATP or ADP, SpoIIAA can undergo two alternative reactions. When ATP is present, SpoIIAA is phosphorylated rapidly and completely to SpoIIAA–phosphate, and SpoIIAB is immediately released; but in the presence of ADP, SpoIIAA forms a long-lasting complex with SpoIIAB. ADP is an inhibitor of the phosphorylation by ATP. Furthermore, we have mutated SpoIIAA at residue Ser 58, the target for phosphorylation, to aspartate or alanine. SpoIIAAS58D, which apparently resembles SpoIIAA–phosphate, is unable to make a complex with SpoIIAB and is devoid of anti-anti-σF activity, whereas SpoIIAAS58A, which cannot be phosphorylated, makes complexes with SpoIIAB in the presence of ADP or ATP and has constitutive anti-anti-σF activity both in vivo and in vitro. It seems likely that the alternative reactions of SpoIIAA and SpoIIAB, involving ADP or ATP, regulate the anti-anti-σ capacity of SpoIIAA and hence the activity of σF.