Author:
Reding Katie,Chen Mengyao,Lu Yong,Jarvela Alys M. Cheatle,Pick Leslie
Abstract
AbstractThe discovery of pair-rule genes (PRGs) inDrosophilarevealed the existence of an underlying two-segment-wide prepattern directing embryogenesis. The milkweed bugOncopeltus, a hemimetabolous insect, is a more representative arthropod: most of its segments form sequentially after gastrulation. Here we report the expression and function of orthologs of the complete set of nineDrosophilaPRGs inOncopeltus. SevenOf-PRG-orthologs are expressed in stripes in the primordia of every segment, rather than every-other segment,Of-runtis PR-like, and several are also expressed in the segment addition zone. RNAi-mediated knockdown ofOf-odd-skipped,pairedandsloppy-pairedimpacted all segments, with no indication of PR-like register. We confirm thatOf-E75Ais expressed in PR-like stripes, although it is not PR inDrosophila, demonstrating the existence of an underlying PR-like prepattern inOncopeltus. These findings reveal that a switch occurred in regulatory circuits leading to segment formation: while several holometabolous insects are “Drosophila-like,” utilizing PRG-orthologs for PR-patterning, mostOf-PRGs are expressed segmentally inOncopeltus, a more basally-branching insect. Thus, an evolutionarily stable phenotype – segment formation – is directed by alternate regulatory pathways in diverse species.Summary StatementDespite the broad of conservation of segmentation in insects, the regulatory genes underlying this process inDrosophilahave different roles in the hemipteran,Oncopeltus fasciatus.
Publisher
Cold Spring Harbor Laboratory
Cited by
2 articles.
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