Author:
Zhang Yaoyao,Yan Zhiqiang,Qin Qingyuan,Nisenblat Vicki,Yu Yang,Wang Tianren,Lu Cuiling,Yang Ming,Yang Shuo,Yao Ying,Zhu Xiaohui,Xia Xi,Dang Yujiao,Ren Yixin,Yuan Peng,Li Rong,Liu Ping,Guo Hongyan,Han Jinsong,He Haojie,Wu Yu,Li Meng,Zhang Kun,Wang Yiting,Qiao Jie,Yan Jie,Yan Liying
Abstract
SUMMARYFolliculogenesis is a highly regulated process that involves bidirectional interactions of the oocytes and surrounding granulosa cells (GCs). Little is unknown, however, about the transcriptomic profiles of human oocytes and GCs throughout folliculogenesis. Here we performed a high resolution RNA-Seq of human oocytes and GCs at each follicular stage, which revealed unique transcriptional profiles, stage-specific signature genes, oocyte- and GC-derived genes that reflect ovarian reserve. We identified reciprocal cell-to-cell interactions between oocytes and GCs, including NOTCH, TGF-β signaling and gap junctions and determined the expression patterns of maternal-effect genes involved in folliculogenesis and early embryogenesis. Finally, we demonstrated robust differences between human and mice oocyte transcriptomes. This is the first comprehensive overview of the transcriptomic signatures governing the stepwise human folliculogenesis in-vivo that provides a valuable resource for basic and translational research in human reproductive biology.
Publisher
Cold Spring Harbor Laboratory
Cited by
2 articles.
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