Genotyping-by-sequencing and DNA array for genomic prediction in soybean oil composition

Abstract

AbstractSoybean oil is intended for various purposes, such as cooking oil and biodiesel. The oil composition changes the shelf life, palatability, and how healthy this oil is for the human diet. Genomic selection jointly uses these traits, phenotypes, and markers from one of the available genotyping platforms to increase genetic gain over time. This study aims to evaluate the impact of different genotyping platforms, DNA arrays, and Genotyping-by-Sequencing (GBS) on genomic selection in relation to the composition of fatty acids in soybean oil and total oil content. We used different quality control parameters, such as heterozygote rate, minor allele frequency, and missing data rate in ten combinations, and two prediction models, BayesB and BRR. To compare the impact of the genotyping approaches, we calculated the principal components analysis from the kinship matrices, the SNP density, and the traits prediction accuracies for each approach. Principal component analysis showed that the DNA array explained better the population genetic architecture.On the other hand, prediction accuracies varied between the different genotyping platforms and only GBS was affected under different quality control parameters. Although the DNA array has important and well-studied polymorphisms for soybeans and is stable, it also has ascertainment bias. GBS, although not stable and requires more robust quality control, can discover alleles specific to the population under study. As soybean oil is used for different functions and the fatty acid profiles are different for each objective, the work constitutes a critical study and direction for improving the composition of soybean oil.