Decomposition of L-glutamine and accumulation of ammonium in cell culture media inhibit infectivity of influenza viruses

Author:

Kegel Nathanael B.ORCID,Kaufmann Andreas,Matrosovich Mikhail,Bauer Stefan,Dorna Jens

Abstract

AbstractCationic lysosomotropic molecules such as ammonium salts and chloroquine inhibit influenza A virus (IAV) infection in cell culture by counteracting endosomal acidification and hampering viral-endosomal fusion. Here, we studied the effects of storage of L-glutamine-supplemented cell culture media on accumulation of ammonium and inhibition of IAV infection. The storage-related inhibitory effect was observed in the case of DMEM and OptiMEM media, but not RPMI medium, and was more pronounced for the IAV with pH-stable hemagglutinin. Our results highlight the importance to consider potential presence of virus-inhibiting lysosomotropic agents and/or their production in culture media with negative effect on influenza virus infection.

Publisher

Cold Spring Harbor Laboratory

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