Serosurveillance of dengue infection and correlation with mosquito pools for dengue virus positivity during the COVID-19 pandemic in Tamil Nadu, India – A state-wide cross-sectional cluster randomized community-based study

Author:

Selvavinayagam Sivaprakasam T.ORCID,Sankar SathishORCID,Yong Yean K.ORCID,Anshad Abdul R.,Chandramathi Samudi,Somasundaram Anavarathan,Palani Sampath,Kumarasamy Parthipan,Azhaguvel Roshini,Kumar Ajith B.,Subramaniam Sudharshini,Malathi Manickam,Vijayalakshmi Venkatachalam,Rajeshkumar Manivannan,Kumaresan Anandhazhvar,Pandey Ramendra P.ORCID,Muruganandam NagarajanORCID,Gopalan NatarajanORCID,Kannan MeganathanORCID,Murugesan AmudhanORCID,Balakrishnan PachamuthuORCID,Byrareddy Siddappa N.ORCID,Dash Aditya P.ORCID,Larsson MarieORCID,Velu VijayakumarORCID,Shankar Esaki M.ORCID,Raju Sivadoss

Abstract

SummaryBackgroundDengue is a vector-borne viral disease impacting millions across the globe. Nevertheless, akin to many other diseases, reports indicated a decline in dengue incidence and seroprevalence during the COVID-19 pandemic (2020-22). This presumably could be attributed to reduced treatment-seeking rates, under-reporting, misdiagnosis, disrupted health services and reduced exposure to vectors due to lockdowns. Scientific evidence on dengue virus (DENV) disease during the COVID-19 pandemic is limited globally.MethodsA cross-sectional, randomized cluster sampling community-based survey was carried out to assess anti-dengue IgM and IgG and SARS-CoV-2 IgG seroprevalence across all 38 districts of Tamil Nadu, India. The prevalence of DENV in the Aedes mosquito pools during 2021 was analyzed and compared with previous and following years of vector surveillance for DENV by real-time PCR.FindingsResults implicate that both DENV-IgM and IgG seroprevalence and mosquito viral positivity were reduced across all the districts. A total of 13464 mosquito pools and 5577 human serum samples from 186 clusters were collected. Of these, 3·76% of mosquito pools were positive for DENV. In the human sera, 4·12% were positive for DENV IgM and 6·4% were positive for DENV IgG. The anti-SARS-CoV-2 antibody titres correlated with dengue seropositivity with a significant association whereas vaccination status significantly correlated with dengue IgM levels.InterpretationContinuous monitoring of DENV seroprevalence, especially with the evolving variants of the SARS-CoV-2 virus and surge in COVID-19 cases will shed light on the transmission and therapeutic attributes of dengue infection.

Publisher

Cold Spring Harbor Laboratory

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