Toxicological Analysis of Poly-Lactide Co-Glycolide Microparticles, Chitosan Nanoparticles and Poly(Anhydride) Nanoparticles in Vero Cell

Abstract

ABSTRACTThe present study was carried out to evaluate in-vitro toxicity associated with chitosan nanoparticles, poly(anhydride) nanoparticles and poly-lactide co-glycolide (PLG) microparticle in Vero cell line. The cytotoxicity of all three micro/nano-particles was assessed using different concentration. For each concentration, the confluent monolayer was treated for a period of 36-48 hours and studied for morphological alteration, live and death count etc. after the treatment. It was observed that the different concentrations of chitosan nanoparticles and Gantrez® nanoparticles did not have significant effect on the cell viability as evident from the non-significant difference between the OD540 of formazan product formed from MTT in treated and untreated cells. The concentration of chitosan nanoparticles and Gantrez® nanoparticles up to 1000 μg/ml did not have any influence in cellular metabolic activities and viability. However, a reduction in the cellular viability and metabolic activities were observed when PLG microparticles were used at 1000 μg/ml. At concentrations below 1000 μg/ml, all the nanoparticles/microparticles were found to be safe in terms of cytotoxicity.