Mechanical constraints influence microtubule orientation in plant cells

Abstract

ABSTRACTIn growing plant cells, parallel ordering of microtubules (MTs) along the inner surface of the cell membrane influences the direction of cell expansion and thereby plant morphology. For correct expansion of organs that primarily grow by elongating, such as roots and stems, MTs must bend in the high-curvature direction along the cylindrically shaped cell membrane in order to form the required circumferential arrays. Previous studies, which have recapitulated the self-organization of these arrays, ignored MT mechanics and assumed MTs follow geodesics of the cell surface. Here, we show, through analysis of a derived Euler-Lagrange equation, that an elastic MT constrained to a cylindrical surface will deflect away from geodesics and toward low curvature directions to minimize bending energy. This occurs when the curvature of the cell surface is relatively high for a given anchor density. In the limit of infinite anchor density, MTs always follow geodesics. We compare our analytical predictions to measured curvatures and anchor densities and find that the regime in which cells are forming these cortical arrays straddles the region of parameter space in which arrays must form under the antagonistic influence of this mechanically induced deflection. Although this introduces a potential obstacle to forming circumferentially orientated arrays that needs to be accounted for in the models, it also raises the question of whether plants use this mechanical phenomenon to regulate array orientation. The model also constitutes an elegant generalization of the classical Euler-bucking instability along with an intrinsic unfolding of the associated pitchfork bifurcation.SIGNIFICANCE STATEMENTThe cortical microtubule array in plant cells is a dynamic structure whose organization influences cell function, growth, and plant morphology. During cell growth, the array must be highly ordered and perpendicular to the axis of expansion. In other instances, it can be disordered or axially oriented. Experimental and modelling studies have outlined the importance of various microtubule-microtubule interactions in the ordering and orienting process. We explore the influence of cell shape on the bending of microtubules that has been largely ignored to date. We find that cell shape can induce microtubule bending that steers them away from high-curvature directions. This bending force can act as an obstacle to proposed mechanisms for array organization and also as a mechanism for regulating reorientation of the array.