Author:
Rosspopoff Olga,Huret Christophe,Collier Amanda J.,Casanova Miguel,Rugg-Gunn Peter J.,Ouimette Jean-François,Rougeulle Claire
Abstract
SUMMARYX chromosome inactivation (XCI) is a developmental regulatory process that initiates with remarkable diversity in various mammalian species. Here we addressed the contribution of XCI regulators, most of which are lncRNA genes characterized in the mouse, to this mechanistic diversity. By combining analysis of single-cell RNA-seq data from early human embryogenesis with various functional assays in naïve and primed pluripotent stem cells and in differentiated cells, we demonstrate that JPX is a major regulator of XIST expression in human and in mouse. However, the underlying mechanisms differ radically between species and require Jpx RNA in the mouse and the act of transcription of JPX locus in the human. Moreover, biogenesis of XIST is affected at different regulatory steps between these species. This study illustrates how diversification of LRGs modes of action during evolution provide opportunities for innovations within constrained gene regulatory networks.Graphical abstract
Publisher
Cold Spring Harbor Laboratory
Cited by
2 articles.
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