Image Scanning Microscopy with Single-Photon Detector Array

Author:

Castello Marco,Tortarolo GiorgioORCID,Buttafava MauroORCID,Deguchi TakahiroORCID,Villa FedericaORCID,Koho SamiORCID,Bianchini Paolo,Sheppard Colin J. R.,Diaspro AlbertoORCID,Tosi AlbertoORCID,Vicidomini GiuseppeORCID

Abstract

Image scanning microscopy (ISM) improves the spatial resolution of conventional confocal laser-scanning microscopy (CLSM), but current implementations reduce versatility and restrict its combination with fluorescence spectroscopy techniques, such as fluorescence lifetime. Here, we describe a natural design of ISM based on a fast single-photon detector array, which allows straightforward upgrade of an existing confocal microscope, without compromising any of its functionalities. In contrast to all-optical ISM implementations, our approach provides access to the raw scanned images, opening the way to adaptive reconstruction methods, capable of considering different imaging conditions and distortions. We demonstrate its utility in the context of fluorescence lifetime, deep, multicolor and live-cell imaging. This implementation will pave the way for a transparent and massive transition from conventional CLSM to ISM.confocal microscopy | time-resolved spectroscopy | image scanning microscopy | single-photon detector array

Publisher

Cold Spring Harbor Laboratory

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