Adaptive stress response induced by toluene increasesSporothrix schenckiivirulence and host immune response

Abstract

ABSTRACTEnvironmental factors modify the physiology of microorganisms, allowing their survival in extreme conditions. However, the influence of chemical contaminants on fungal virulence has been little studied. Sporotrichosis is an emergent fungal disease caused bySporothrix schenckii,a soil-inhabiting fungus that has been found in polluted environments. Here, we evaluated the adaptive stress response ofS. schenckiiinduced by toluene, a key soil contaminant. The effect on fungal virulence and host immune response was also assessed. The fungus survived up to 0.10% toluene in liquid medium. Greater production of melanosomes and enhanced activity superoxide dismutase, associated to increased tolerance to H2O2were observed in toluene-exposed fungi. Intraperitoneal infection of mice withS. schenckiitreated with either 0, 0.01 or 0.1% of toluene, resulted in greater fungal burden at day 7 post-infection in spleen and liver in the groups infected with fungus treated with toluene 0.1%. A higher production of Il-1β, TNF-α, IL-10 and nitric oxyde by peritoneal macrophages and IFNγ, IL-4 and IL-17 by splenocytes was also observed in that group. Our findings showed that morphological and functional changes induced by toluene leads to increasedS. schenckiivirulence and antifungal host immune response in our model.