Author:
Rosenberg Marjorie,Hui Lester,Ma Junli,Nusbaum Harris C.,Clark Kevin,Robinson Louise,Dziadzio Laura,Swain Pamela M.,Keith Tim,Hudson Thomas J.,Biesecker Leslie G.,Flint Jonathan
Abstract
Completion of genetic and physical maps requires markers from the ends (telomeres) of every human chromosome. We have searched for short tandem repeats (microsatellites) in cosmid and P1 clones and generated 661 sequence-tagged sites (STS) from the terminal 300 kb of 31 human chromosome ends. PCR assays were successfully designed for 58 microsatellites and mapped both genetically and on radiation hybrids (RHs) to confirm their telomeric location. Sequence analysis revealed marked variation in sequence composition, consistent with the hypothesis that even very highly GC-rich chromosome bands (the T bands) are not homogenous. The STSs that we have generated will be a necessary resource for the construction of physical maps of these complex regions of the genome.[Information about the microsatellites is available electronically at http://www.cshl.org/gr and sequence has been deposited in the Genome Database (GDB).]
Publisher
Cold Spring Harbor Laboratory
Subject
Genetics(clinical),Genetics
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