Author:
Ma Ning,de Mochel Nabora Reyes,Pham Paula Duyen Anh,Yoo Tae Yeon,Cho Ken WY.,Digman Michelle A.
Abstract
AbstractDevelopment of quantitative, safe and rapid techniques for assessing embryo quality provides significant advances in Assisted Reproductive Technologies (ART). We apply the phasor-FLIM method to capture endogenous fluorescent biomarkers of pre-implantation embryos as a non-morphological caliber for embryo quality. Here, we identify the developmental, or “D-trajectory”, that consists of fluorescence lifetime from different stages of mouse pre-implantation embryos. The D-trajectory correlates with intrinsic fluorescent species from a distinctive energy metabolism and oxidized lipids, as seen with Third Harmonic Generation (THG) that changes over time. In addition, we have defined an Embryo Viability Index (EVI) to distinguish pre-implantation embryo quality using the Distance Analysis, a machine learning algorithm to process the fluorescence lifetime distribution patterns. We show that the phasor-FLIM approach provides a much-needed non-invasive quantitative technology for identifying healthy embryos at the early compaction stage with 86% accuracy. This may increase embryo implantation success for in vitro fertilization clinics.HighlightsA label-free method of tracking metabolic trajectories during pre-implantation mouse embryo development.A non-invasive approach for assessing embryo quality and viability by a phasor-FLIM analysis.
Publisher
Cold Spring Harbor Laboratory
Cited by
1 articles.
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