Author:
L’Amoreaux Nicholas,Ali Aon,Iqbal Shoaib,Larsen Jessica
Abstract
AbstractSelf-assembled polymersomes encapsulate, protect, and deliver hydrophobic and hydrophilic drugs. Though spherical polymersomes are effective, early studies suggest that non-spherical structures may enhance specificity of delivery and uptake due to similarity to endogenous uptake targets. Here we describe a method to obtain persistent non-spherical shapes, prolates, via osmotic pressure and the effect of prolates on uptake behavior. Polyethylene glycol-b-poly(lactic acid) polymersomes change in diameter from 175 ± 5nm to 200 ± 5nm and increase in polydispersity from 0.06 ± 0.02 to 0.122 ± 0.01 nm after addition of 50 mM salt. Transmission and scanning electron microscopy confirm changes from spheres to prolates. Prolate-like polymersomes maintain their shape in 50 mM NaCl for seven days. Nile Red and bovine serum albumin(BSA)-Fluorescein dyes are taken up in greater amounts by SH-SY5Y neural cells when encapsulated in polymersomes. Prolate polymersomes may be taken up more efficiently in neural cells than spherical polymersomes.
Publisher
Cold Spring Harbor Laboratory
Reference46 articles.
1. Larsen, J. M. ; Martin, D. R. ; Byrne, M. E. Recent Advances in Delivery through the Blood-Brain Barrier. Curr. Top. Med. Chem. 2014, No. 14, 1148–1160.
2. Pardridge, W. M. Drug Delivery to the Brain. J. Cereb. Blood Flow Metab. 1997, No. 17, 713–731.
3. Barriers to carrier mediated drug and gene delivery to brain tumors
4. Tsuji, A. ; Tamai, I. Blood-Brain Barrier Transport of Drugs. In Introduction to the Blood-Brain Barrier: Methodology, Biology, and Pathology2; Pardridge, W. M. , Ed.; Cambridge University Press, 2006; pp 238–245.
5. Blood–brain barrier delivery