Rational programming of history-dependent logic in cellular populations

Author:

Zúñiga AnaORCID,Guiziou SarahORCID,Mayonove Pauline,Meriem Zachary Ben,Camacho Miguel,Moreau ViolaineORCID,Ciandrini LucaORCID,Hersen PascalORCID,Bonnet JeromeORCID

Abstract

AbstractGenetic programs operating in an history-dependent fashion are ubiquitous in nature and govern sophisticated processes such as development and differentiation. The ability to systematically and predictably encode such programs would advance the engineering of synthetic organisms and ecosystems with rich signal processing abilities. Here we implement robust, scalable history-dependent programs by distributing the computational labor across a cellular population. Our design is based on recombinase-driven DNA scaffolds expressing different genes according to the order of occurrence of inputs. These multicellular computing systems are highly modular and any program can be built by differential composition of strains containing well-characterized logic scaffolds. We developed an automated workflow that researchers can use to streamline program design and optimization. We anticipate that the history-dependent programs presented here will support many applications using cellular populations for material engineering, biomanufacturing and healthcare.One Sentence SummarySystematic and automated frameworks for implementing robust history-dependent genetic programs in cellular populations.

Publisher

Cold Spring Harbor Laboratory

Cited by 1 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. Engineering Synthetic Signaling in Plants;Annual Review of Plant Biology;2020-04-29

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