Massive transient damage of the olfactory epithelium associated with infection of sustentacular cells by SARS-CoV-2 in golden Syrian hamsters
Author:
Bryche Bertrand,St Albin Audrey,Murri Severine,Lacôte Sandra,Pulido Coralie,Gouilh Meriadeg Ar,Lesellier Sandrine,Servat Alexandre,Wasniewski Marine,Picard-Meyer Evelyne,Monchatre-Leroy Elodie,Volmer Romain,Rampin Olivier,Le Goffic Ronan,Marianneau Philippe,Meunier Nicolas
Abstract
AbstractAnosmia is one of the most prevalent symptoms of SARS-CoV-2 infection during the COVID-19 pandemic. However, the cellular mechanism behind the sudden loss of smell has not yet been investigated. The initial step of odour detection takes place in the pseudostratified olfactory epithelium (OE) mainly composed of olfactory sensory neurons surrounded by supporting cells known as sustentacular cells. The olfactory neurons project their axons to the olfactory bulb in the central nervous system offering a potential pathway for pathogens to enter the central nervous system by bypassing the blood brain barrier. In the present study, we explored the impact of SARS-COV-2 infection on the olfactory system in golden Syrian hamsters. We observed massive damage of the OE as early as 2 days post nasal instillation of SARS-CoV-2, resulting in a major loss of cilia necessary for odour detection. These damages were associated with infection of a large proportion of sustentacular cells but not of olfactory neurons, and we did not detect any presence of the virus in the olfactory bulbs. We observed massive infiltration of immune cells in the OE and lamina propria of infected animals, which may contribute to the desquamation of the OE. The OE was partially restored 14 days post infection. Anosmia observed in COVID-19 patient is therefore likely to be linked to a massive and fast desquamation of the OE following sustentacular cells infection with SARS-CoV-2 and subsequent recruitment of immune cells in the OE and lamina propria.
Publisher
Cold Spring Harbor Laboratory
Reference26 articles.
1. Bilinska, K. , Jakubowska, P. , CS, V.O.N.B. , Butowt, R. , 2020. Expression of the SARS-CoV-2 Entry Proteins, ACE2 and TMPRSS2, in Cells of the Olfactory Epithelium: Identification of Cell Types and Trends with Age. ACS Chem Neurosci. 2. Neurologic Alterations Due to Respiratory Virus Infections;Frontiers in cellular neuroscience,2018 3. Bryche, B. , Fretaud, M. , Saint-Albin Deliot, A. , Galloux, M. , Sedano, L. , Langevin, C. , Descamps, D. , Rameix-Welti, M.A. , Eleouet, J.F. , Le Goffic, R. , Meunier, N. , 2019b. Respiratory syncytial virus tropism for olfactory sensory neurons in mice. J Neurochem, e14936. 4. Bryche, B. , Dewaele, A. , Saint-Albin, A. , Le Poupon Schlegel, C. , Congar, P. , Meunier, N. , 2019a. IL-17c is involved in olfactory mucosa responses to Poly(I:C) mimicking virus presence. Brain, behavior, and immunity. 5. Corman, V.M. , Landt, O. , Kaiser, M. , Molenkamp, R. , Meijer, A. , Chu, D.K. , Bleicker, T. , Brunink, S. , Schneider, J. , Schmidt, M.L. , Mulders, D.G. , Haagmans, B.L. , van der Veer, B. , van den Brink, S. , Wijsman, L. , Goderski, G. , Romette, J.L. , Ellis, J. , Zambon, M. , Peiris, M. , Goossens, H. , Reusken, C. , Koopmans, M.P. , Drosten, C. , 2020. Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR. Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin 25.
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