Anin-vitroBBB-on-a-chip open model of human blood-brain barrier enabling advanced optical imaging

Author:

Salman Mootaz,Marsh Graham,Küsters Ilja,Delincé Matthieu,Di Caprio Giuseppe,Upadhyayula Srigokul,de Nola Giovanni,Hunt Ronan,Ohashi Kazuka G.,Shimizu Fumitaka,Sano Yasuteru,Kanda Takashi,Obermeier Birgit,Kirchhausen TomORCID

Abstract

ABSTRACTWe describe here the design and implementation of anin-vitroBBB-on-a-chip open model system capable of reconstituting the microenvironment of the blood brain barrier. This system allows controlled unidirectional flow of nutrients and biologicals on the lumen of the artificial microvessel. This BBB-on-a-chip is suitable for high resolution electron microscopy and it is amenable for quantitative 3D live fluorescence imaging using spinning confocal disk or lattice light sheet microscopy (LLSM) to follow, for example the transcytosis across the BBB-like barrier of fluorescently-tagged biological, viruses or nanoparticles.

Publisher

Cold Spring Harbor Laboratory

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