Population Dynamics and Neuronal Polyploidy in the Developing Neocortex

Abstract

SUMMARYThe mammalian neocortex is composed of different subtypes of neurons which are generated during embryogenesis by sequential differentiation of neural progenitors. While molecular mechanisms that control neuronal production in the developing neocortex have been extensively studied, the dynamics and absolute numbers of the different progenitor and neuronal populations are still poorly characterized. Here we describe a medium throughput approach based on flow cytometry and well known identity markers of cortical subpopulations to collect quantitative data over the course of mouse neocortex development. We collected a complete dataset in a physiological developmental context on two progenitor and two neuron populations, including relative proportions and absolute numbers. Our study reveals unexpected numbers of progenitors. In addition, we discovered that a fraction of neurons in the developing mouse neocortex are polyploid.