Bright Near-infrared Anti-Stokes Fluorescence of ICG under Low Power CW Laser Excitation and its Applications in Bioimaging

Abstract

AbstractAnti-Stokes fluorescence was observed in ICG, a molecule approved by the FDA for clinical use. The wavelengths of its fluorescence are mainly located in the near-infrared band of 800 nm~900 nm, with a high quantum yield up to 8%. In order to know its generation mechanism, based on multi-photon absorption (MPA) theory, thermally activated delayed fluorescence (TADF) theory and hot band absorption theory, its power dependence, temperature dependence of absorption spectra and fluorescence spectra, and fluorescence lifetime were measured. Its generation mechanism was finally determined to be hot band absorption process. Since ICG showed bright anti-Stokes fluorescence in near-infrared region, which offers substantially longer penetration depth in biological tissues than visible light, excellent photostability and biosafety, we applied it to in vivo imaging and compared it with upconversion nanoparticles (UCNPs). The result is that ICG exhibited much stronger fluorescence than UCNPs, providing more anatomical information of samples. This contributes to a better choice for anti-Stokes fluorescence bioimaging.