Author:
Lahmy Sylvie,Pontier Dominique,Bies-Etheve Natacha,Laudié Michèle,Feng Suhua,Jobet Edouard,Hale Christopher J.,Cooke Richard,Hakimi Mohamed-Ali,Angelov Dimitar,Jacobsen Steven E.,Lagrange Thierry
Abstract
RNA polymerase V (Pol V) long noncoding RNAs (lncRNAs) have been proposed to guide ARGONAUTE4 (AGO4) to chromatin in RNA-directed DNA methylation (RdDM) in plants. Here, we provide evidence, based on laser UV-assisted zero-length cross-linking, for functionally relevant AGO4–DNA interaction at RdDM targets. We further demonstrate that Pol V lncRNAs or the act of their transcription are required to lock Pol V holoenzyme into a stable DNA-bound state that allows AGO4 recruitment via redundant glycine–tryptophan/tryptophan–glycine AGO hook motifs present on both Pol V and its associated factor, SPT5L. We propose a model in which AGO4–DNA interaction could be responsible for the unique specificities of RdDM.
Funder
Agence Nationale de la Recherche
Centre National de la Recherche Scientifique
Université de Perpignan Via Domitia
European Union's Seventh Framework Programme
National Institutes of Health
Publisher
Cold Spring Harbor Laboratory
Subject
Developmental Biology,Genetics