ePat: extended PROVEAN annotation tool

Abstract

AbstractThe ‘ePat’ (extended PROVEAN annotation tool) is a software tool that extends the functionality of PROVEAN: a software tool for predicting whether amino acid substitutions and indels will affect the biological function of proteins. The ‘ePat’ extends the conventional PROVEAN to enable the following two things, which the conventional PROVEAN could not calculate the pathogenicity of these variants. First is to calculate and score the pathogenicity of indel mutations with frameshift and variants near splice junctions. Second is to use batch processing to calculate the pathogenicity of multiple variants into a variants list (VCF file) in a single step. ePat can help extract variants that affect biological functions by utilizing not only point mutations, and indel mutations that does not cause frameshift, but also frameshift, stop gain, and splice variants. These extended features will increase detection rate and improve diagnostic of inherited diseases or associate specific variant to phenotype.