Evaluation of the Performance of Filmarray Blood Culture Identification Panel on Detecting Blood Cultures Containing Activated Carbon Powder

Abstract

AbstractObjectiveThe FilmArray Blood Culture Identification (BCID) panel is a rapid microfluidic PCR amplification microbial detection system. Several studies have evaluated its clinical performance on the basis of blood culture bottles containing resins. However, proportion of hospitals in China use bottles with carbon power, which the performance of FilmArray has not been fully investigated. Therefore, this study is conducted to explore the accuracy of the panel using blood culture bottles with carbon power.Method147 venous blood cultures containing carbon powder were used to assess the microbial and antibiotic resistance detection ability of the FilmArray panel. Outcomes were compared with results of the clinical combination method and their consistency was analyzed.ResultsFilmArray detected single microorganism in 121 samples, multiple microorganism in 9 cases and the consistency rate between the two methods was 90.6%. Among the 150 microorganisms detected, 85.1% (40/47) of staphylococcus contained the antibiotic resistant mecA gene, 15.3% (9/59) of Enterobacter detected the KPC gene, 7.7% (1/13) of Enterococcus has the vanA gene and the consistency with their clinical drug-resistant phenotypes were 93.6%, 86.4% and 100%, respectively.ConclusionThe identification rate of the FilmArray BCID panel using venous blood cultures with activated carbon powder was highly consistent with the outcomes of previous researchers using non-carbon powder blood culture bottles. It is capable of providing rapid and reliable results in the detection of pathogens present in automated blood culture systems.