The Basic Polymerase Chain Reaction (PCR)-Reference-Cited by-同舟云学术

The Basic Polymerase Chain Reaction (PCR)

Published:2018-05 Issue:5 Volume:2018 Page:pdb.prot095117
ISSN:1940-3402
Container-title:Cold Spring Harbor Protocols
language:en
Short-container-title:Cold Spring Harb Protoc

Author:

Green Michael R.,Sambrook Joseph

Abstract

This protocol describes the reagents and procedures required to amplify a segment of double-stranded DNA in a chain reaction catalyzed by a thermostable DNA polymerase. It is the foundation for all subsequent variations of the polymerase chain reaction (PCR).

Publisher

Cold Spring Harbor Laboratory

Subject

General Biochemistry, Genetics and Molecular Biology

Reference11 articles.

1. The fidelity of Taq polymerase catalyzing PCR is improved by an N-terminal deletion

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5. Improved cloning efficiency of polymerase chain reaction (PCR) products after proteinase K digestion

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