Assessing the Immune Response When Raising Antibodies for Use in Xenopus

Author:

Piccinni Maya Z.,Guille Matthew J.ORCID

Abstract

Frog-specific antibodies usually must be raised for work in Xenopus. Selecting a host animal whose immune system will respond to a target antigen with an antibody response is essential to obtaining high-quality antibodies. To determine whether an immunized animal has produced antibodies against an antigen, western blotting using Xenopus embryo or egg extract as the protein source can be performed as described here. When a protein of the expected size is detected by western blotting in the immune sera but not the preimmune sera, the antibody has been successfully raised.

Publisher

Cold Spring Harbor Laboratory

Subject

General Biochemistry, Genetics and Molecular Biology

Cited by 3 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. The Use of Xenopus for Cell Biology Applications;Cold Spring Harbor Protocols;2021-03-29

2. Purifying Antibodies Raised against Xenopus Peptides;Cold Spring Harbor Protocols;2020-01-03

3. Raising Antibodies for Use in Xenopus;Cold Spring Harbor Protocols;2020-01-03

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