Whole-Mount In Situ Hybridization in Amphimedon-Reference-Cited by-同舟云学术

Whole-Mount In Situ Hybridization in Amphimedon

Published:2008-12 Issue:12 Volume:2008 Page:pdb.prot5096
ISSN:1940-3402
Container-title:Cold Spring Harbor Protocols
language:en
Short-container-title:Cold Spring Harb Protoc

Author:

Larroux Claire,Fahey Bryony,Adamska Maja,Richards Gemma S.,Gauthier Marie,Green Kathryn,Lovas Erica,Degnan Bernard M.

Abstract

INTRODUCTIONDevelopmental gene expression is analyzed predominantly via whole-mount in situ hybridization using digoxigenin-labeled RNA probes. This protocol describes how to perform this procedure in Amphimedon queenslandica, including fixation, hybridization, and sectioning of embryonic, larval, and post-larval juvenile stages.

Publisher

Cold Spring Harbor Laboratory

Subject

General Biochemistry, Genetics and Molecular Biology

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