Mounting Caenorhabditis elegans Embryos for Live Imaging of Embryogenesis: Figure 1.

Author:

Bao Zhirong,Murray John I.

Abstract

INTRODUCTIONCaenorhabditis elegans has been a key model organism for biomedical research. Light microscopy has played a central role in C. elegans biology. C. elegans is transparent throughout its life cycle, and its physical size, from 50 µm (embryos) to 1 mm (adults), is well suited for light microscopy. Furthermore, it has an invariant body plan that arises from an invariant cell lineage. A wide range of biological processes, from patterns of gene expression to cell migration to neuronal activity, can be readily observed in single cells with a well-defined developmental context. This protocol describes how to collect and mount young C. elegans embryos for live imaging throughout embryogenesis.

Publisher

Cold Spring Harbor Laboratory

Subject

General Biochemistry, Genetics and Molecular Biology

Reference3 articles.

1. Hutter H . 2006. Fluorescent reporter methods. In C. elegans: Methods applications (ed. Strange K ), pp. 155–174. Humana Press, Totowa, NJ.

2. Schnabel R . 1999. Microscopy. In C. elegans: A practical approach (ed. Hope IA ), pp. 119–142. Oxford University Press, New York.

3. The embryonic cell lineage of the nematode Caenorhabditis elegans

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