Infrared Video Microscopy for Visualizing Neurons and Neuronal Excitation in Brain Slices-Reference-Cited by-同舟云学术

Infrared Video Microscopy for Visualizing Neurons and Neuronal Excitation in Brain Slices

Published:2013-12 Issue:12 Volume:2013 Page:pdb.prot079434
ISSN:1940-3402
Container-title:Cold Spring Harbor Protocols
language:en
Short-container-title:Cold Spring Harb Protoc

Author:

Dodt Hans-Ulrich,Becker Klaus,Zieglgänsberger Walter

Abstract

Here we describe methods for imaging neurons and neuronal excitation in brain slices with infrared video microscopy. Patch clamping in conjunction with a gradient-contrast system allows electrical recording from fine neuronal processes. Imaging with an infrared (IR)-darkfield system allows visualization of the intrinsic optical signal (IOS).

Publisher

Cold Spring Harbor Laboratory

Subject

General Biochemistry, Genetics and Molecular Biology

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