Dissection of Embryonic Mouse Kidney, Culture In Vitro, and Imaging of the Developing Organ

Author:

Costantini Frank,Watanabe Tomoko,Lu Benson,Chi Xuan,Srinivas Shankar

Abstract

INTRODUCTIONIn this article, we outline procedures for the dissection of intact kidneys and the isolation of the ureteric bud (UB) and the metanephric mesenchyme (MM) from mouse embryos. The apparatus required for the culture of these tissues in vitro is described in detail as well as the equipment necessary for performing time-lapse imaging studies of the developing kidney.

Publisher

Cold Spring Harbor Laboratory

Subject

General Biochemistry, Genetics and Molecular Biology

Reference6 articles.

1. Imaging Kidney Development

2. A method for cold storage and transport of viable embryonic kidney rudiments;Kidney Int,2006

3. Hogan B , Beddington R , Costantini F , Lacey E . 1994. Manipulating the mouse embryo: A laboratory manual, 2nd ed. p. 151. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.

4. Branching morphogenesis independent of mesenchymal-epithelial contact in the developing kidney

5. Multiple fibroblast growth factors support growth of the ureteric bud but have different effects on branching morphogenesis

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