Abstract
INTRODUCTIONThis protocol describes a method for quantitating the amount of free thiols and disulfide bond linkages in proteins. It uses the sulfhydryl reagent 5,5′-dithiobis (2-nitrobenzoic acid), or DTNB, which is also referred to as Ellman’s reagent.
Publisher
Cold Spring Harbor Laboratory
Subject
General Biochemistry, Genetics and Molecular Biology
Cited by
13 articles.
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