Abstract
INTRODUCTIONThis protocol presents methods for the specific detection of neuronal cell-surface receptors and secreted factors. These techniques allow visualization of surface pools of receptor proteins and also reveal the surface distribution of secreted cues. Live dissection of embryos to expose the ventral nerve cord is also described. This procedure can be used to improve the ability to detect signals from suboptimal antibodies, where conventional staining gives poor results.
Publisher
Cold Spring Harbor Laboratory
Subject
General Biochemistry, Genetics and Molecular Biology
Cited by
4 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献