Phenotypic Analysis of Preimplantation Mouse Embryos in Culture
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Published:2023-11-06
Issue:12
Volume:2023
Page:pdb.prot108090
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ISSN:1940-3402
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Container-title:Cold Spring Harbor Protocols
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language:en
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Short-container-title:Cold Spring Harb Protoc
Author:
Papaioannou Virginia E.,Behringer Richard R.
Abstract
Preimplantation embryo culture is a valuable approach to investigate a preimplantation lethal phenotype. Standard culture methods have been perfected such that the entire preimplantation period and the process of implantation can be followed in vitro. This protocol provides modifications for the analysis of clutches of embryos from heterozygous matings specifically for the purpose of distinguishing a preimplantation phenotype in homozygous mutants.
Publisher
Cold Spring Harbor Laboratory
Subject
General Biochemistry, Genetics and Molecular Biology
Reference5 articles.
1. Behringer RR , Gertsenstein M , Nagy KV , Nagy A . 2014. Manipulating the mouse embryo: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
2. Administration of Gonadotropins for Superovulation in Mice