Author:
Tisi Renata,Martegani Enzo,Brandão Rogelio L.
Abstract
A photoelectric flame photometer is a device used in inorganic chemical analysis for determining the concentrations of certain metals in solution. It does this by measuring the intensity of the light emitted by the metal when the solution is sprayed under controlled conditions into a nonluminous flame. This protocol describes how to measure total cellular calcium (maximal emission at 622 nm, orange flame) in yeast using this technique.
Publisher
Cold Spring Harbor Laboratory
Subject
General Biochemistry, Genetics and Molecular Biology