In Vitro Fertilization of the African Turquoise KillifishNothobranchius furzeri

Abstract

The ability to perform in vitro fertilization, together with sperm cryopreservation, greatly facilitates the long-term laboratory maintenance of wild-type and transgenic model organisms and helps prevent genetic drift. It is also useful in cases where reproduction may be compromised. In this protocol, we present a method for in vitro fertilization of the African Turquoise killifishNothobranchius furzerithat is compatible with the use of fresh or cryopreserved sperm.