High-Throughput Measurement of the Ca2+-Dependent ATPase Activity in COS Microsomes-Reference-Cited by-同舟云学术

High-Throughput Measurement of the Ca2+-Dependent ATPase Activity in COS Microsomes

Published:2014-08 Issue:8 Volume:2014 Page:pdb.prot076885
ISSN:1940-3402
Container-title:Cold Spring Harbor Protocols
language:en
Short-container-title:Cold Spring Harb Protoc

Author:

Vandecaetsbeek Ilse,Holemans Tine,Wuytack Frank,Vangheluwe Peter

Abstract

We provide a detailed procedure to determine the Ca2+-dependent ATPase activity in COS or HEK293 cells overexpressing a Ca2+ pump. The ATPase activity is determined by the Baginsky method, which allows measurement of the steady-state production of inorganic phosphate (Pi). We have adapted this widely applied method into a sensitive, fast, and semi-high-throughput protocol suitable for use in a 96-well plate format.

Publisher

Cold Spring Harbor Laboratory

Subject

General Biochemistry, Genetics and Molecular Biology

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