Author:
Beisson Janine,Bétermier Mireille,Bré Marie-Hélène,Cohen Jean,Duharcourt Sandra,Duret Laurent,Kung Ching,Malinsky Sophie,Meyer Eric,Preer John R.,Sperling Linda
Abstract
INTRODUCTIONParamecium has a highly complex cellular structure. The cytoskeleton of these organisms interacts with cilia at the cell surface to control cell movement, feeding, and environmental responses. Basal bodies anchored in the cortex organize the cytoskeleton and determine cell shape, and a conspicuous cytoskeletal network, the infraciliary lattice, underlies the cell surface and plays an important role in cellular Ca2+ homeostasis. This protocol describes methods to visualize the cytoskeleton, especially cortical structures. It is not directly applicable for visualization of membrane proteins.
Publisher
Cold Spring Harbor Laboratory
Subject
General Biochemistry, Genetics and Molecular Biology
Cited by
13 articles.
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