Parthenogenetic Activation of Oocytes

Author:

Kaufman Matthew H.

Abstract

Numerous studies have been initiated to investigate the influence of maternal and paternal genomes on early mammalian development. For this type of study, parthenogenetic embryos provide a unique source of preimplantation and early postimplantation embryos that (by definition) develop in the absence of any contribution from a male gamete. Parthenogenetic activation is used for biochemical and morphological studies of oocytes during fertilization and early development and is a critical component of the cloning procedure. This protocol describes the activation of oocytes using ethanol. Parthenogenesis can also be induced by exposure of unfertilized oocytes to strontium-containing medium.

Publisher

Cold Spring Harbor Laboratory

Subject

General Biochemistry, Genetics and Molecular Biology

Reference8 articles.

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5. The chromosome complement of single-pronuclear haploid mouse embryos following activation by ethanol treatment;J Embryol Exp Morphol,1982

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