Author:
Galione Antony,Chuang Kai-Ting,Funnell Tim M.,Davis Lianne C.,Morgan Anthony J.,Ruas Margarida,Parrington John,Churchill Grant C.
Abstract
NAADP and other Ca2+-mobilizing messengers are membrane impermeant and thus must be added directly to cell-free or broken-cell preparations to effect Ca2+ release. The sea urchin egg homogenate, where the biological activity of NAADP was first reported, remains the gold standard cell-free system for studying NAADP-mediated Ca2+ release. Here we describe how to prepare sea urchin egg homogenate and use it to measure NAADP-mediated Ca2+ release.
Publisher
Cold Spring Harbor Laboratory
Subject
General Biochemistry, Genetics and Molecular Biology
Cited by
6 articles.
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