Direct Injection of Indicators for Calcium Imaging at the Drosophila Larval Neuromuscular Junction: Figure 1.

Author:

Macleod Gregory T.

Abstract

Calcium imaging is a technique in which Ca2+-binding molecules are loaded into live cells and as they bind Ca2+ they “indicate” the concentration of free calcium through a change in either the intensity or the wavelength of light emitted (fluorescence or bioluminescence). There are several possible methods for loading synthetic Ca2+ indicators into subcellular compartments, including topical application of membrane-permeant Ca2+ indicators, forward-filling of dextran conjugates, and direct injection. Calcium imaging is a highly informative technique in neurobiology because Ca2+ is involved in many neuronal signaling pathways and serves as the trigger for neurotransmitter release. This article describes the direct injection of Ca2+ indicators at the Drosophila larval neuromuscular junction (NMJ). This technique allows rapid loading of most Ca2+ indicators, but there are drawbacks in that it is a difficult technique to master and requires additional electrophysiological equipment. Also, Ca2+ indicators that are easily injected are usually susceptible to compartmentalization.

Publisher

Cold Spring Harbor Laboratory

Subject

General Biochemistry, Genetics and Molecular Biology

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3