AnophelesAdult Anesthesia, Feeding, and Sex Separation

Abstract

The adult stage is the only nonaquatic stage of theAnophelesmosquito. Both male and femaleAnophelesmosquitoes require access to a source of sugar to survive. In the insectary, a temperature of ∼27°C and 80% relative humidity and a cycle of 12 h light:12 h dark light, ideally with a sunrise and sunset period, are necessary minimum conditions to mimic their natural environment. Laboratory-rearedAnophelescan survive for over a month; however, decreased activity and increased mortality may be observed ∼2 wk postemergence depending on the species and health of the colony. Details on how to maintain adultsAnophelesare discussed here. Information and considerations on blood and sugar feeding are described. This protocol also provides instructions on how to differentiate male and female adult mosquitoes.