Author:
Clayton David A.,Shadel Gerald S.
Abstract
Rat or mouse liver is the most frequently used tissue for mitochondrial preparations because it is readily available, easy to homogenize, and replete with mitochondria. A motor-driven Teflon and glass Potter-Elvehjem homogenizer is the best choice for homogenizing liver, but if one is not available, this tissue is soft enough that a Dounce homogenizer with a loose (A) pestle can also be used. The yield and purity of the mitochondrial preparation will be influenced by the method and speed of preparation and the age and physiological condition of the animal.
Publisher
Cold Spring Harbor Laboratory
Subject
General Biochemistry, Genetics and Molecular Biology
Reference3 articles.
1. Organelle isolation: functional mitochondria from mouse liver, muscle and cultured filroblasts
2. Rickwood D . 1992. Preparative centrifugation: A practical approach. IRL Press, Oxford.
3. Tyler DD . 1992. The mitochondrion in health and disease. VCH, New York.
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