Author:
Reece-Hoyes John S.,Walhout Albertha J.M.
Abstract
High-efficiency yeast transformation is used for integrations into YM4271 (yeast one-hybrid (Y1H) DNA-bait generation), for transforming libraries of activation domain (AD)-prey clones into Y1H and yeast two-hybrid (Y2H)-bait strains, and for gap repair. The protocol takes 2 d to complete.
Publisher
Cold Spring Harbor Laboratory
Subject
General Biochemistry, Genetics and Molecular Biology