Isotype Determination of Rodent-Derived Monoclonal Antibodies Using Sandwich ELISA
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Published:2017-11
Issue:11
Volume:2017
Page:pdb.prot093831
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ISSN:1940-3402
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Container-title:Cold Spring Harbor Protocols
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language:en
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Short-container-title:Cold Spring Harb Protoc
Author:
Weis-Garcia Frances,Carnahan Robert H.
Abstract
For research groups needing to isotype a significant number of antibodies on a fairly regular basis, the protocol outlined here provides a cost-effective option. In this simple sandwich ELISA, the monoclonal antibody is first captured with antibodies that discriminate between heavy-chain isotypes and then is detected with antibodies specific for each light chain. This combination ensures that free light chain secreted by hybridomas does not yield a false-positive result, because in a true positive both the capture and detecting antibodies bind the monoclonal antibody.
Publisher
Cold Spring Harbor Laboratory
Subject
General Biochemistry, Genetics and Molecular Biology
Cited by
1 articles.
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1. Characterizing Antibodies;Cold Spring Harbor Protocols;2017-11