Author:
Green Michael R.,Sambrook Joseph
Abstract
In this protocol, hybridization is first performed in conventional aqueous solvents at a temperature well below the melting temperature, and the hybrids are then washed at higher stringency in buffers containing quaternary alkylammonium salts. TMACl is used with probes that are 14–50 nt in length, whereas TEACl is used with oligonucleotides that are 50–200 nt in length.
Publisher
Cold Spring Harbor Laboratory
Subject
General Biochemistry, Genetics and Molecular Biology
Cited by
1 articles.
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