Isolation and Sequencing of NovelVibrioSpecies

Abstract

Whole-genome sequencing of bacteria facilitates their genotypic analysis and expands our understanding of the tree of life on Earth. TheVibriogenus comprises many halophilic species of bacteria, including some that are pathogenic to humans and other animals. Here I describe methods for isolating and sequencing both known and novel species ofVibriofrom saltwater environments. The first section outlines methods of isolating and phenotypically characterizing strains, followed by purification of their total DNA. The second and third sections outline methods of whole-genome sequencing and assembly, annotation, and phylogenetic analysis.