Staphylococcus aureusColony Polymerase Chain Reaction

Abstract

Here, we describe a protocol for a colony polymerase chain reaction (PCR) method forStaphylococcus aureus. The methodology involves the preparation of smallS. aureuslysates by using the enzyme lysostaphin to degrade the peptidoglycan layer. These lysates are prepared using a small patch of bacteria grown on LB agar plates, and the lysates can subsequently be used for PCR analyses.