Preparation and Orcein Staining of Mitotic Chromosomes from Drosophila Larval Brain-Reference-Cited by-同舟云学术

Preparation and Orcein Staining of Mitotic Chromosomes from Drosophila Larval Brain

Published:2010-03 Issue:3 Volume:2010 Page:pdb.prot5389
ISSN:1940-3402
Container-title:Cold Spring Harbor Protocols
language:en
Short-container-title:Cold Spring Harb Protoc

Author:

Pimpinelli Sergio,Bonaccorsi Silvia,Fanti Laura,Gatti Maurizio

Abstract

INTRODUCTIONIn this protocol, larval brains from Drosophila are incubated in vitro with colchicine, treated with hypotonic solution, fixed, and squashed in aceto-orcein. This procedure provides a large number of well-spread metaphase figures (200-400 per brain) that can be analyzed for chromosome morphology, the presence of chromosome aberrations, and the degree of ploidy.

Publisher

Cold Spring Harbor Laboratory

Subject

General Biochemistry, Genetics and Molecular Biology

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