Isolation and whole genome characterization of antagonisticPaenibacillus polymyxa188 and its biocontrol potential against several fungi

Abstract

AbstractDiscovery of new antifungal compounds and biocontrol agents is important due to the emergence of drug-resistant fungi and new fungal pathogens. The aim of this study was to isolate marine bacteria that are able to produce metabolites with antifungal properties. In this study, we isolated aP. polymyxa,named as 188, from a marine sediment sample and evaluated its antifungal ability. The results indicated that the bacterium showed excellent antifungal activity against many pathogenic fungi of plants and humans. The antifungal compounds produced byP. polymyxa188 were extracted and analyzed using MALDI-TOF/MS. The complete genome sequence and biosynthetic gene clusters were characterized, and further to compare the genomes ofP. polymyxa188 with other strains. Various CAZymes were identified inP. polymyxa188. Five antibiotic gene clusters including paenilan, paenibacillin, fusaricidin, polymyxin, and tridecaptin can be found inP. polymyxastrains, but gene clusters of paenilan, paenibacillin, and polymyxin were absent inP. polymyxa188. Our findings provided detail genetic information aboutP. polymyxa188 and suggested thatP. polymyxa188 is the potential agent of biocontrol and disease management.