The conserved protein CBA1 is required for vitamin B12uptake in different algal lineages

Abstract

AbstractMicroalgae play an essential role in global net primary productivity and global biogeochemical cycling, but despite their phototrophic lifestyle, over half of algal species depend on a supply of the corrinoid vitamin B12(cobalamin) for growth. This essential organic micronutrient is produced only by a subset of prokaryotic organisms, which implies that for algal species to use this compound, they must first acquire it from external sources. Previous studies have identified protein components involved in vitamin B12uptake in bacterial species and humans. However, little is known about how it is taken up in algae. Here, we demonstrate the essential role of a protein, CBA1 (for cobalamin acquisition protein 1), in B12uptake inPhaeodactylum tricornutum, using CRISPR-Cas9 to generate targeted knockouts, and inChlamydomonas reinhardtii, by insertional mutagenesis. In both cases, CBA1 knockout lines are no longer able to take up exogenous vitamin B12. Complementation of theC. reinhardtiimutants with the wildtypeCBA1gene restores B12uptake, and regulation ofCBA1expression via a riboswitch element can be used to control the phenotype. When visualised by confocal microscopy, a YFP-fusion withC. reinhardtiiCBA1 shows association with membranes. A bioinformatics analysis found that CBA1-like sequences are present in all the major eukaryotic phyla. Its presence is correlated with B12-dependent enzymes in many, although not all, taxa, suggesting CBA1 has a conserved role. Our results thus provide insight into the molecular basis of algal B12acquisition, a process that likely underpins many interactions in aquatic microbial communities.One sentence summaryKnockout mutants and physiological studies demonstrate that the CBA1 protein is essential for uptake of vitamin B12in bothChlamydomonas reinhardtiiand the unrelatedPhaeodactylum tricornutum.