Author:
da Silva Jean Paulo Aparecido,Viana José Marcelo Soriano,Dias Kaio Olimpio das Graças,Silva Jéssica Coutinho,Tupper Vivian Torres Bandeira,Clarindo Wellington Ronildo
Abstract
AbstractFor popcorn, obtaining and identifying haploids are still challenging steps. We aimed to induce and screen haploids in popcorn using the Navajo phenotype, seedling vigor and ploidy level. We used the Krasnodar Haploid Inducer (KHI) in crosses with 20 popcorn source germplasms and five maize controls. The field trial design was completely randomized, with three replications. We assessed the efficacy of induction and of identification of haploids based on haploidy induction rate (HIR) and false positive and negative rates (FPR and FNR). Additionally, we also measured the penetrance of the Navajo marker gene (R1-nj). All putative haploids classified byR1-njwere germinated together with a diploid sample and evaluated for false positives and negatives based on vigor. Seedlings from 14 females were submitted to flow cytometry to determine the ploidy level. The HIR and penetrance were analyzed by fitting a generalized linear model with a logit link function. The HIR of the KHI, adjusted by cytometry, ranged from 0.0 to 1.2%, with a mean of 0.34%. The average FPR from screening based on the Navajo phenotype were 26.2% and 76.4%, by the vigor and ploidy, respectively. The FNR was zero. The penetrance ofR1-njranged from 30.8 to 98.6%. The average number of seeds per ear in temperate germplasm (76) was lower than that obtained in tropical germplasm (98). There is induction of haploids in germplasm of tropical and temperate origin. We recommend the selection of haploids associating the Navajo phenotype with a direct method of confirming the ploidy level, such as flow cytometry. We also show that haploid screening based on Navajo phenotype and seedling vigor reduces misclassification. The origin and genetic background of the source germplasm influence theR1-njpenetrance. Because the known inducers are maize, developing doubled-haploid technology for popcorn hybrid breeding requires overcoming the unilateral cross-incompatibility.
Publisher
Cold Spring Harbor Laboratory