Hiding in Plain Sight: Phylogenomics Reveals a New Branch on the Noctuoidea Tree of Life

Abstract

AbstractWe present the results of the first phylogenomic analyses based on anchored hybrid enrichment (AHE) data from densely sampled tribes and subfamilies of Notodontidae (Prominent Moths). Our analyses reveal the family’s polyphyly with respect to an assemblage of genera related toScranciaHolland that has been variously recognized at the tribal or subfamilial rank. We propose and re-describe Scranciidae,stat. nov., and recognize 21 genera and approximately 100 species—distributed in Africa, Asia, and Australia and not represented in previous phylogenomic studies—from the six recognized noctuoid families (Noctuidae, Erebidae, Euteliidae, Nolidae, Notodontidae, and Oenosandridae). We further re-interpret morphological synapomorphies previously proposed for Notodontidae (including Scranciidae) and for the trifid Noctuoidea,viz.the ventral-facing tympanum and trifid forewing venation— characters previously called into question when Doidae were transferred from Noctuoidea to Drepanoidea. Deep-level relationships within Noctuoidea are not firmly established outside the clade comprising the four quadrifid families (Noctuidae, Erebidae, Euteliidae, and Nolidae), and in attempting to establish the phylogenetic position of Scranciidae relative to Notodontidae, Oenosandridae, and the quadrifids, we obtain conflicting results depending on data type (amino acid vs. nucleotide) and analytical framework (maximum likelihood, multi-species coalescent, and parsimony). We also demonstrate that discordant topologies among these ancient lineages yield drastically different divergence time estimates, highlighting the need for caution when interpreting phylogenetic dating of uncertain topologies. Following multiple analyses of several datasets designed around the distribution of missing data, and an evaluation of strict support measures at the deepest nodes of the noctuoid tree, we provisionally conclude that this ambiguity is a function of character conflict amplified by missing data and short branch lengths, and that in the topology best supported by the available data, Scranciidae is placed well outside Notodontidae and sister to the remaining Noctuoidea.